Class-Specific Protein Assay Kits
Abcam SIRT1 Activity Assay Kit (Fluorometric)
SIRT1 Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Raybiotech Inc Pyroglutamyl-Peptidase I Human Recombinant 20ug
Pyroglutamyl-Peptidase I Human Recombinant 20ug
Abcam MMP-3 Activity Assay Kit (Fluorometric)
MMP-3 Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
AnaSpec SENSOLYTE 520 TEV ACTIVITY KIT
SensoLyte 520 TEV Activity Assay Kit *Fluorimetric*, (1 kit) Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 Deg.C, making it the protease of choice for fusion proteins or tags removal. The SensoLyte TEV Protease Assay Kit uses TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly proportional to the TEV protease quantity/activity.
XpressBio HIV-1 Integrase Wild-Type Assay
The HIV-1 Integrase Assay Kit is a non-radioactive assay used to quantitatively measure integrase activity, the effects of interacting proteins, anti-viral compounds, and other test articles on HIV-1 integrase activity.
Enzo Life Sciences Auto-ubiquitinylation kit (1x10tests)
This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control. Contents: 20X Ubiquitin Activating Enzyme Solution (E1), 25µl. 20X Ubiquitin Conjugating Enzyme Solution (E2), 25µl. 10X Ubiquitin Solution (Ub), 50µl. 20X Control Ubiquitin Ligase Solution (E3), 25µl. 20X Mg-ATP Solution, 25µl. 10X Ub E3 ligase Buffer, 50µl. Ubiquitin antibody solution, 25µl
TRIBIOSCIENCE INC Tryptase Activity Assay, 100 Tests
The Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for the evaluation of tryptase activity in cell lysates, supernatants, or for inhibitor screening.
TOPOGEN INC Topoisomerase I Assay Kit 500 Assays Includes Top1
This kit contains reagents for routine detection of type I topoisomerase activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking of the supercoiled substrate (although we note that some eukaryotic type I activities are stimulated by Mg++). This assay will work even with crude extracts.
AnaSpec SENSOLYTE 520 MOUSE RENIN ASS
SensoLyte 520 Mouse Renin Assay Kit Fluorimetric, (1 kit) Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I. Angiotensin I is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research. The SensoLyte 520 Mouse Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of mouse renin activity using a 5-FAM/QXL520 FRET peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm.
Kamiya Biomedical LIPID HYDROPEROXIDE KIT LPO-CC
LPO-CC (Lipid Peroxides) Kit. 165 mL. For the quantitative colorimetric determination of high levels of lipid peroxides in serum, plasma, and other biological samples. Assay range = 2.0 - 300 nmol/mL. (165 tests with 1 mL test tube, 550 tests with 96-well microtiter plate). Includes calibrator.
Cell Biolabs Inc OxiSelect™ Catalase Activity Assay Kit (Colorimetric), 100 assays
Hydrogen peroxide is a reactive oxygen species that is a toxic product of normal aerobic metabolism and pathogenic ROS production. Hydrogen peroxide is poisonous to eukaryotic cells and in high doses can initiate oxidation of DNA, lipids, and proteins, which can lead to mutagenesis and cell death. Catalase is an antioxidant enzyme omnipresent in mammalian and non-mammalian cells that destroys hydrogen peroxide by dismutation. The OxiSelect™ Catalase Activity Assay is a fast and reliable kit for the direct measurement of catalase activity from cell lysate, plasma, serum, whole blood, and tissue homogenates. Direct spectrophotometric detection of catalase activity with ultraviolet light can cause interference from proteins and other biological components. The assay utilizes visible light (520 nm), which reduces sample interference. Each kit provides sufficient reagents to perform up to 96 assays in a microtiter plate including blanks, catalase standards and unknown samples.